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Relative expression of osteogenic genes during osteogenic differentiation: (a) collagen I ( COL1A1 ) at days 4, 7, and 14; (b) alkaline phosphatase ( ALPL ), (c) <t>osteopontin</t> ( <t>SPP1</t> ), and (d) osteocalcin ( BGLAP ) at days 4, 7, 14, 21, and 28 in the studied hydrogels Col I, Col I/II-HA, Col I/II-HA+Cho, and Col I/II-HA+Cho_only. Mean of 2 −ΔΔCt + SD (n = 3). Expression compared to day 0 of the corresponding hydrogel, set as 1 (not shown on the graph). Two-way ANOVA with Tukey’s multiple comparisons test ( P ≤ 0.05). Comparisons for each time interval separately; significance: $ in comparison with Col I/II-HA, # in comparison with Col I/II-HA+Cho, and * in comparison with Col I/II-HA+Cho_only.
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Relative expression of osteogenic genes during osteogenic differentiation: (a) collagen I ( COL1A1 ) at days 4, 7, and 14; (b) alkaline phosphatase ( ALPL ), (c) <t>osteopontin</t> ( <t>SPP1</t> ), and (d) osteocalcin ( BGLAP ) at days 4, 7, 14, 21, and 28 in the studied hydrogels Col I, Col I/II-HA, Col I/II-HA+Cho, and Col I/II-HA+Cho_only. Mean of 2 −ΔΔCt + SD (n = 3). Expression compared to day 0 of the corresponding hydrogel, set as 1 (not shown on the graph). Two-way ANOVA with Tukey’s multiple comparisons test ( P ≤ 0.05). Comparisons for each time interval separately; significance: $ in comparison with Col I/II-HA, # in comparison with Col I/II-HA+Cho, and * in comparison with Col I/II-HA+Cho_only.
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Relative expression of osteogenic genes during osteogenic differentiation: (a) collagen I ( COL1A1 ) at days 4, 7, and 14; (b) alkaline phosphatase ( ALPL ), (c) osteopontin ( SPP1 ), and (d) osteocalcin ( BGLAP ) at days 4, 7, 14, 21, and 28 in the studied hydrogels Col I, Col I/II-HA, Col I/II-HA+Cho, and Col I/II-HA+Cho_only. Mean of 2 −ΔΔCt + SD (n = 3). Expression compared to day 0 of the corresponding hydrogel, set as 1 (not shown on the graph). Two-way ANOVA with Tukey’s multiple comparisons test ( P ≤ 0.05). Comparisons for each time interval separately; significance: $ in comparison with Col I/II-HA, # in comparison with Col I/II-HA+Cho, and * in comparison with Col I/II-HA+Cho_only.

Journal: Cell Transplantation

Article Title: Collagen-hyaluronic acid hydrogel with embedded chondrocytes as a platform for modeling early stages of endochondral ossification in vitro

doi: 10.1177/09636897251409464

Figure Lengend Snippet: Relative expression of osteogenic genes during osteogenic differentiation: (a) collagen I ( COL1A1 ) at days 4, 7, and 14; (b) alkaline phosphatase ( ALPL ), (c) osteopontin ( SPP1 ), and (d) osteocalcin ( BGLAP ) at days 4, 7, 14, 21, and 28 in the studied hydrogels Col I, Col I/II-HA, Col I/II-HA+Cho, and Col I/II-HA+Cho_only. Mean of 2 −ΔΔCt + SD (n = 3). Expression compared to day 0 of the corresponding hydrogel, set as 1 (not shown on the graph). Two-way ANOVA with Tukey’s multiple comparisons test ( P ≤ 0.05). Comparisons for each time interval separately; significance: $ in comparison with Col I/II-HA, # in comparison with Col I/II-HA+Cho, and * in comparison with Col I/II-HA+Cho_only.

Article Snippet: For osteogenic cell differentiation, the following genes were examined: Col I ( COL1A1 gene, Hs00164004_m1), an early differentiation marker; alkaline phosphatase ( ALPL gene, Hs01029144_m1), an intermediate marker; osteocalcin ( BGLAP gene, Hs00165814_m1) and osteopontin ( SPP1 gene, Hs00959010_m1), late differentiation markers.

Techniques: Expressing, Comparison